Biochemistry

Protein stability is another common problem in protein expression. It is also an important topic in purification, formulation, and storage. Here we will discuss protein stability in expression only. Properly folded proteins are usually stable during expression and purification. Sufficient amount of intact protein should be obtained. However, some proteins …

When discussing protein fold we first need to identify the folding unit. Such unit is called a protein domain. This means that when we talk about fold classification we actually mean PROTEIN DOMAIN CLASSIFICATION. Protein domains are the basic building blocks of a protein, Structural Organization of Proteins and they …